m00356 1  (Boster Bio)

Journal: Journal of Translational Medicine

Article Title: A dynamic molecular landscape in colorectal cancer progression at single-cell resolution

doi: 10.1186/s12967-025-06785-9

Figure Lengend Snippet: Tumor-like epithelial cells identified in epithelial cells from CRC tissues. A The UMAP plot displaying the annotation of four sub-types of epithelial cells with corresponding color codes. B A UMAP plot illustrating the distribution of the four sub-cell types of epithelial cells across the four types of colorectal samples, with red arrows indicating specific cell types within the corresponding sample types. C InferCNV analyses shown in the violin plot indicate the genomic variation and malignancy across all cell types, with a red arrow highlighting the epithelial cells. Epithelial cells of the four sub-types ( D ) and from each sample type ( E ) are depicted in developmental trajectory analyses. Tumor-like epithelial cells from CRC tissues are highlighted in the red dashed square. Feature plots of BEST4 + ( F ) and CEACAM5 + ( G ) expression in epithelial cells are highlighted by the red arrows, respectively

Article Snippet: CEACAM5 , Anti-CEA (CD66e) CEACAM5 Rabbit Monoclonal Antibody , Boster , M00356-1 , 1:100.

Techniques: Expressing

Journal: Journal of Translational Medicine

Article Title: A dynamic molecular landscape in colorectal cancer progression at single-cell resolution

doi: 10.1186/s12967-025-06785-9

Figure Lengend Snippet: Immunofluorescence staining visualizes BEST4 + OTOP2 + and tumor-like epithelial cells in colorectal tissues. A Combined IF staining for CLDN4 (green channel, pan-marker gene for epithelial cells) and BEST4 (red channel) demonstrating the presence of BEST4 + epithelial cells in the four types of clinical colorectal samples, predominant in normal tissues. B Combined IF staining for CLDN4 (green channel) and CEACAM5 (red channel, pan-cancer cancer marker gene) showing the presence of CEACAM5 + epithelial cells, enriched in CRC tissure. Nuclei were counter-stained with DAPI. Tissues within the white dashed squares were imaged at higher magnification as displayed on the right side. Each staining was performed in triplicates using tissue slides sourced from three different individuals. A scale bar is present in each image

Article Snippet: CEACAM5 , Anti-CEA (CD66e) CEACAM5 Rabbit Monoclonal Antibody , Boster , M00356-1 , 1:100.

Techniques: Immunofluorescence, Staining, Marker